NPB
Study ID

Study Title

Principle
Investigator

Objective(s)

Determine the environmental stability of PEDv:
1. To determine survival of PEDv in fresh feces that represents the risk posed by transport.
2. To determine survival of PEDv in slurry (old feces in the pit) that reflects the risk of manure spreading.
3. To study PEDv survival in drinking and recycled water (truck washes).
4. To study PEDv survival in animal feed.

1. Prevalence and incidence of PED virus contamination in transport vehicles at slaughter.
2. Determine incidence of infection in unrelated sites adjacent to known infected sites and identify site-level risk factors associated with infection.

12/12/2013

1. Development and validation of diagnostic testing for PEDv antigen and antibody detection.
1.2. Development of standardized reference samples that can be utilized by veterinary diagnostic labs (VDLs) for diagnostic test validation.
1.3. Develop and validate antibody-based diagnostic tests for serologic monitoring and surveillance.

1. Determine if oral fluids can be sample matrix to detect PEDv and/or virus-specific antibody.
2. Evaluate the performance of PEDv diagnostics on oral fluid samples.
3. Assess the utility of oral fluid sampling and testing for PEDv monitoring on-farm.

The objective of this study is to investigate the combinations of time and temperature sufficient to kill PEDv in swine feces on the metal surface of a model trailer used to haul live pigs. The results of this study will inform producers and veterinarians about the adquacy of curent sanitation procedures for live-haul trailers and enable them to make better decisions about investments in truck sanitation.

Study of the basic pathogenesis and characterization of the virus: Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of PEDv following inoculation of feeder pigs will be investigated. In an attempt to expand diagnostic testing capabilities, multiple aliquots of all samples will be collected and shared with requesting laboratories.

1. Development and standardization of viral propagation techniques to produce virus for use in diagnostic testing (outcome to be publicly available to all VDLs for PEDv diagnostics).
2. Development of standardized reference samples that can be utilized by VDLs for diagnostic test validation.
3. Validation of the current PCR diagnostic tests.

1. Develop and validate antibody-based diagnostic tests for serologic monitoring and surveillance.
2. Develop and validate oral fluid testing (antigen and antibody testing capabilities) to provide rapid method for sample collection and to support on-farm surveillance.