Antigenic Characterization of Novel H1 Influenza A Viruses in Swine
April 24, 2020 —
Novel H1N2 influenza A viruses (IAVs) in swine have been identified in Chile co-circulating with pandemic H1N1 2009-like (A(H1N1)pdm09-like) viruses. The objective of this study was to characterize antigenically the swine H1 IAVs circulating in Chile. Genetic analysis based on the HA1 domain and antigenic analysis by hemagglutination inhibition assay were carried out. Three antigenic clusters were identified, named Chilean H1 A (ChH1A), Chilean H1 B (ChH1B), and A(H1N1)pdm09-like. The antigenic sites of ChH1A and ChH1B strains were 10-60% distant from those of commercial vaccine strains at the amino acid sequence level. Antigenic variants were identified within the clusters ChH1A and A(H1N1)pdm09-like. Substitutions in the main antigenic sites (E153G in Sa, Q193H in Sb, D168N in Ca1, P137S in Ca2, and F71L in Cb) were detected in variants from the ChH1A cluster, whereas only a single substitution in antigenic site Sa (G155E) was detected in variants from A(H1N1)pdm09-like cluster, which confirms the importance to carrying out antigenic analyses in addition to genetic analyses to evaluate control measures such as vaccination. These results highlight the need to update vaccines for swine in Chile and the importance of continued surveillance to determine the onward transmission of antigenic variants in Chilean pig populations.
Tapia R, Torremorell M, Culhane M, Medina R, Neira V. Antigenic characterization of novel H1 influenza A viruses in swine. Sci Rep. 2020 Mar. https://doi.org/10.1038/s41598-020-61315-5
- Next story: Oral Vitamin A Supplementation of Porcine Epidemic Diarrhea Virus Infected Gilts Enhances IgA and Lactogenic Immune Protection of Nursing Piglets [Abs.]
- Previous in category: Influence of Setback Distance on Antibiotics and Antibiotic Resistance Genes in Runoff and Soil Following the Land Application of Swine Manure Slurry [Abs.]
- Previous story: AASV Staff Working Remotely Through April [AASV]