Restriction fragment length polymorphism analysis following unexpected Mycoplasma hyopneumoniae diagnosis in a gilt multiplication herd
J. C. S. Harding, DVM, MSc, Diplomate ABVP
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Lung tissue from multiple pigs from a serologically negative gilt multiplication barn unexpectedly tested positive for Mycoplasma hyopneumoniae by polymerase chain reaction (PCR) and fluorescent antibody test. While sporadic coughing, dyspnea, and unthriftiness were noted on initial examination, the clinical presentation was atypical of mycoplasmal pneumonia. A detailed diagnostic investigation, including a follow-up visit, examination at slaughter, serological testing, additional necropsies, PCR, and restriction fragment length polymorphism analysis, confirmed the herd’s M hyopneumoniae-negative status. This report highlights the importance of a thorough clinical and diagnostic examination following an unexpected positive laboratory result. Moreover, the excellent feedback and collaboration with the diagnostic laboratory in this case facilitated the continuous improvement of laboratory services for the mutual benefit of the laboratory’s clientele.
Keywords: Mycoplasma hyopneumoniae, polymerase chain reaction, PCR, restriction fragment length polymorphism, RFLP, diagnosis
Cite as: Harding J C S. Restriction fragment length polymorphism analysis following unexpected Mycoplasma hyopneumoniae diagnosis in a gilt multiplication herd. J Swine Health Prod 2008;16(5):247-253.
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