Identification of Trueperella abortisuis contamination in extended boar semen

Eva Bussalleu, MS, PhD; Gary C. Althouse, MS, PhD, DVM, Diplomate ACT

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Trueperella abortisuis is a gram-positive bacterium that has been previously identified in aborted porcine feti and placentae located in Asia and Europe. Routine microbiological screening of extended boar semen from a US mid-Atlantic stud identified delayed growth of very small white colonies on both brain-heart infusion and sheep-blood agars after 48 to 72 hours incubation at 37°C under aerobic conditions. Isolate identification was performed using matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry, with T abortisuis identified (MALDI biotyper score = 2.103). After storage at 16°C and 72 hours post collection, total and progressive motility parameters had decreased in extended semen samples positive for T abortisuis. Further work is needed to elucidate the role T abortisuis may play in extended boar semen quality, extended semen longevity, and sow reproductive performance.

Keywords: Trueperella abortisuis, boar, extended semen

RIS citationCite as: Bussalleu E, Althouse GC. Identification of Trueperella abortisuis contamination in extended boar semen. J Swine Health Prod 2017;25(6):299-302.

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