TY - JOUR AU - Harding, J, C, S TI - Restriction fragment length polymorphism analysis following unexpected Mycoplasma hyopneumoniae diagnosis in a gilt multiplication herd T2 - Journal of Swine Health and Production JF - Journal of Swine Health and Production J2 - JSHAP SN - 1537-209X DP - American Association of Swine Veterinarians PB - American Association of Swine Veterinarians DA - 2008/Sep// PY - 2008 VL - 16 M1 - 5 IS - 5 M2 - 247 SP - 247-253 L2 - https://www.aasv.org/shap/issues/v16n5/v16n5p247.htm UR - https://www.aasv.org/shap/abstracts/abstract.php?v16n5p247 L1 - https://www.aasv.org/shap/issues/v16n5/v16n5p247.pdf KW - swine KW - Mycoplasma hyopneumoniae KW - polymerase chain reaction KW - PCR KW - restriction fragment length polymorphism KW - RFLP KW - diagnosis N2 - Lung tissue from multiple pigs from a serologically negativegilt multiplication barn unexpectedly tested positive for Mycoplasma hyopneumoniae by polymerase chain reaction (PCR)and fluorescent antibody test. While sporadic coughing, dyspnea,and unthriftiness were noted on initial examination, the clinicalpresentation was atypical of mycoplasmal pneumonia. A detaileddiagnostic investigation, including a follow-up visit, examinationat slaughter, serological testing, additional necropsies, PCR, andrestriction fragment length polymorphism analysis, confirmed theherd’s M hyopneumoniae-negative status. This reporthighlights the importance of a thorough clinical and diagnosticexamination following an unexpected positive laboratory result.Moreover, the excellent feedback and collaboration with thediagnostic laboratory in this case facilitated the continuousimprovement of laboratory services for the mutual benefit of thelaboratory’s clientele. ER -