TY - JOUR AU - Royer, RL AU - Nawagitgul, P AU - Halbur, PG TI - Susceptibility of porcine circovirus type 2 to commercial and laboratory disinfectants T2 - Journal of Swine Health and Production JF - Journal of Swine Health and Production J2 - JSHAP SN - 1537-209X DP - American Association of Swine Veterinarians PB - American Association of Swine Veterinarians DA - 2001/Nov// PY - 2001 VL - 9 M1 - 6 IS - 6 M2 - 281 SP - 281-284 L2 - https://www.aasv.org/shap/issues/v9n6/v9n6p281.html UR - https://www.aasv.org/shap/abstracts/abstract.php?v9n6p281 L1 - https://www.aasv.org/shap/issues/v9n6/v9n6p281.pdf KW - swine KW - disinfectants KW - porcine circovirus KW - PCV2 N2 - Objective: To evaluate the virucidal efficacy of 11 commercially available disinfectants against porcine circovirus type 2 (PCV2) using an in vitro model.Methods: Disinfectants were prepared according to the manufacturers' label directions and mixed with virus stock. The disinfectant-virus solution was then passed through a detoxification column to remove compounds toxic to cell culture. The filtrate was collected, serially diluted, and inoculated onto porcine kidney cells (PK-15). After a 48-hour incubation period, the cell cultures were fixed and an indirect immunofluorescence assay performed to determine remaining infectious virus titers. Virus titers after disinfection were compared to the negative control (no disinfectant), using Dunnett's test for statistical analysis.Results: Results demonstrated statistically significant reduction in PCV2 virus titer by several disinfectants, including Virkon(R) S, sodium hydroxide, Roccal(R) D Plus, Clorox(R) Bleach, 1-Stroke Environ(R), Fulsan(R), and Tek-Trol(R). No significant reduction in PCV2 titer was demonstrated using Nolvasan(R), DC&R(R), Weladol(R), or ethanol.Implications: This study was performed in vitro under controlled laboratory conditions. Variation from these results may occur under field conditions. Nevertheless, these results may aid in the selection of more effective disinfectants to reduce exposure to PCV2 and the incidence of post-weaning multisystemic wasting syndrome or other PCV2-associated diseases. ER -