Porcine rotavirus groups A, B, and C identified by polymerase chain reaction in a fecal sample collection with inconclusive results by polyacrylamide gel electrophoresis
Kerlei C. MÃ©dici, DVM, PhD; Aline F. Barry, DVM, MSc; Alice F. Alfieri, DVM, PhD; Amauri A. Alfieri, DVM, PhD
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Objective: To evaluate the frequency of groups A, B, and C rotaviruses in diarrheic pigs from Brazilian pig herds.
Materials and methods: Fecal samples with inconclusive results when tested by the silver-stained polyacrylamide gel electrophoresis (ss-PAGE) technique were selected for this study (n = 144). Rotavirus A (VP4 and VP7 genes), rotavirus B (NSP2 gene), and rotavirus C (VP6 gene) were identified by reverse transcription-polymerase chain reaction (RT-PCR).
Results: Of the 144 fecal specimens analyzed by RT-PCR assays, 103 (71.5%) were positive for rotavirus infection. Single infections were detected in 58 samples (40.3%), with 34 (23.6%), 19 (13.2%), and 5 (3.5%) identified as rotavirus groups A, B, and C, respectively. Mixed infections with two and even three rotavirus groups were identified in 45 fecal samples (31.2%). Rotaviruses B and C were more frequently identified in mixed (65.2%) than in single infections.
Implications: The inclusion criteria for sample selection and use of RT-PCR assays for diagnosis in this study contributed to the higher frequencies of rotaviruses B and C, which are sporadically implicated in porcine neonatal diarrhea. The high rate of diagnosis of atypical rotavirus showed that rotaviruses B and C, as well as rotavirus A, are disseminated in Brazilian pig herds. These results suggest that failure to identify porcine rotaviruses B and C in diarrheic fecal samples is primarily due to use of diagnostic methods of low sensitivity, and not to low prevalence of infection.
Keywords: neonatal diarrhea, group A rotavirus, atypical rotaviruses, reverse transcription-polymerase chain reaction, RTPCR
Cite as: MÃ©dici KC, Barry AF, Alfieri AF, et al. Porcine rotavirus groups A, B, and C identified by polymerase chain reaction in a fecal sample collection with inconclusive results by polyacrylamide gel electrophoresis. J Swine Health Prod 2011;19(3):146-150.
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