United States isolates of Lawsonia intracellularis from porcine proliferative enteropathy resemble European isolates
Jeffrey P. Knittel; David I. Larson, DVM; DL Harris, PhD, DVM; Michael B. Roof, PhD; Steven McOrist, PhD, DVM
Objective--To compare isolates of Lawsonia intracellularis from United States pigs to European isolates.
Design and procedure--Three isolates of L. intracellulariswere isolated from the intestines of United States pigs with porcine proliferative enteropathy. The organisms were grown and passaged in a rat enterocyte cell line (IEC-18). Growth was not associated with morphological cell change and was monitored by immunostaining of the cells using a monoclonal antibody and by polymerase chain reaction (PCR). The PCR products were evaluated by DNA sequencing and compared to a known sequence from L. intracellularis.
Results- Lawsonia intracellularis is a microaerophilic, Gram-negative, curved or S-shaped bacillus and is an obligately intracellular organism. These isolates resemble European isolates by PCR, monoclonal antibody reactions, and growth requirements.
Keywords: Lawsonia intracellularis, isolate
Cite as: Knittel JP, Larson DI, Harris DL, et al. United States isolates of Lawsonia intracellularis from porcine proliferative enteropathy resemble European isolates. J Swine Health Prod 1996;4(3):118-122.
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